4BMJ

Structure of the UBZ1and2 tandem of the ubiquitin-binding adaptor protein TAX1BP1

4BMJ の概要

• エントリーDOI: 10.2210/pdb4bmj/pdb
• 分子名称: TAX1-BINDING PROTEIN 1, ZINC ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: apoptosis, ubz domains, ubiquitin, inflammation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 93519.84

構造登録者

Ceregido, M.A.,Spinola-Amilibia, M.,Buts, L.,Rivera, J.,Bravo, J.,van Nuland, N.A.J. (登録日: 2013-05-09, 公開日: 2013-11-20, 最終更新日: 2024-05-08)

主引用文献

Ceregido, M.A.,Spinola-Amilibia, M.,Buts, L.,Rivera-Torres, J.,Garcia-Pino, A.,Bravo, J.,Van Nuland, N.A.J.
The Structure of Tax1BP1 Ubz1 + 2 Provides Insight Into Target Specificity and Adaptability
J.Mol.Biol., 426:674-, 2014

PubMed Abstract: TAX1BP1 is a novel ubiquitin-binding adaptor protein involved in the negative regulation of the NF-kappaB transcription factor, which is a key player in inflammatory responses, immunity and tumorigenesis. TAX1BP1 recruits A20 to the ubiquitinated signaling proteins TRAF6 and RIP1, leading to their A20-mediated deubiquitination and the disruption of IL-1-induced and TNF-induced NF-kappaB signaling, respectively. The two zinc fingers localized at its C-terminus function as novel ubiquitin-binding domains (UBZ, ubiquitin-binding zinc finger). Here we present for the first time both the solution and crystal structures of two classical UBZ domains in tandem within the human TAX1BP1. The relative orientation of the two domains is slightly different in the X-ray structure with respect to the NMR structure, indicating some degree of conformational flexibility, which is rationalized by NMR relaxation data. The observed degree of flexibility and stability between the two UBZ domains might have consequences on the recognition mechanism of interacting partners.

PubMed: 24239949
DOI: 10.1016/J.JMB.2013.11.006

実験手法

X-RAY DIFFRACTION (2.75 Å)