4BJR

Crystal structure of the complex between Prokaryotic Ubiquitin-like Protein Pup and its Ligase PafA

4BJR の概要

• エントリーDOI: 10.2210/pdb4bjr/pdb
• 分子名称: PUP--PROTEIN LIGASE, PROKARYOTIC UBIQUITIN-LIKE PROTEIN PUP, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ligase, prokaryotic proteasome
• 由来する生物種: CORYNEBACTERIUM GLUTAMICUM
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116098.93

構造登録者

Barandun, J.,Delley, C.L.,Ban, N.,Weber-Ban, E. (登録日: 2013-04-19, 公開日: 2013-05-01, 最終更新日: 2023-12-20)

主引用文献

Barandun, J.,Delley, C.L.,Ban, N.,Weber-Ban, E.
Crystal Structure of the Complex between Prokaryotic Ubiquitin-Like Protein Pup and its Ligase Pafa.
J.Am.Chem.Soc., 135:6794-, 2013

PubMed Abstract: Prokaryotic ubiquitin-like protein (Pup) is covalently attached to target proteins by the ligase PafA, tagging substrates for proteasomal degradation. The crystal structure of Pup in complex with PafA, reported here, reveals that a long groove wrapping around the enzyme serves as a docking site for Pup. Upon binding, the C-terminal region of the intrinsically disordered Pup becomes ordered to form two helices connected by a linker, positioning the C-terminal glutamate in the active site of PafA.

PubMed: 23601177
DOI: 10.1021/JA4024012

実験手法

X-RAY DIFFRACTION (2.8 Å)