4BJ4

Structure of Pseudomonas aeruginosa amidase Ampdh2

4BJ4 の概要

• エントリーDOI: 10.2210/pdb4bj4/pdb
• 関連するPDBエントリー: 4BOL 4BPA
• 分子名称: AMPDH2, CITRATE ANION (3 entities in total)
• 機能のキーワード: hydrolase, periplasmic amidase
• 由来する生物種: PSEUDOMONAS AERUGINOSA PAO1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55183.10

構造登録者

Martinez-Caballero, C.S.,Carrasco-Lopez, C.,Artola-Recolons, C.,Hermoso, J.A. (登録日: 2013-04-16, 公開日: 2013-07-24, 最終更新日: 2023-12-20)

主引用文献

Martinez-Caballero, S.,Lee, M.,Artola-Recolons, C.,Carrasco-Lopez, C.,Hesek, D.,Spink, E.E.,Lastochkin, E.,Zhang, W.,Hellman, L.M.,Boggess, B.,Mobashery, S.,Hermoso, J.A.
Reaction Products and the X-Ray Structure of Ampdh2, a Virulence Determinant of Pseudomonas Aeruginosa.
J.Am.Chem.Soc., 135:10318-, 2013

PubMed Abstract: The zinc protease AmpDh2 is a virulence determinant of Pseudomonas aeruginosa, a problematic human pathogen. The mechanism of how the protease manifests virulence is not known, but it is known that it turns over the bacterial cell wall. The reaction of AmpDh2 with the cell wall was investigated, and nine distinct turnover products were characterized by LC/MS/MS. The enzyme turns over both the cross-linked and noncross-linked cell wall. Three high-resolution X-ray structures, the apo enzyme and two complexes with turnover products, were solved. The X-ray structures show how the dimeric protein interacts with the inner leaflet of the bacterial outer membrane and that the two monomers provide a more expansive surface for recognition of the cell wall. This binding surface can accommodate the 3D solution structure of the cross-linked cell wall.

PubMed: 23819763
DOI: 10.1021/JA405464B

実験手法

X-RAY DIFFRACTION (1.722 Å)