4BH8

CRYSTAL STRUCTURE OF GERMLINE ANTIBODY 36-65 IN COMPLEX WITH PEPTIDE GDPRPSYISHLL

4BH8 の概要

• エントリーDOI: 10.2210/pdb4bh8/pdb
• 関連するPDBエントリー: 4BH7
• 分子名称: ANTI-ARS MURINE GERMLINE MONOCLONAL ANTIBODY 36-65, DODECAPEPTIDE ANTIGEN, ... (4 entities in total)
• 機能のキーワード: immune system, germline antibody, antibody polyspecificty
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 48962.38

構造登録者

Khan, T.,Salunke, D.M. (登録日: 2013-03-30, 公開日: 2014-04-16, 最終更新日: 2024-11-13)

主引用文献

Khan, T.,Salunke, D.M.
Adjustable Locks and Flexible Keys: Plasticity of Epitope-Paratope Interactions in Germline Antibodies.
J.Immunol., 192:5398-, 2014

PubMed Abstract: Ag recognition by independent primary Abs against a small flexible Ag with overlapping epitopes was analyzed to address the determinants of Ag specificity during the initial encounter. Crystal structures of two distinct dodecapeptide Ags, GDPRPSYISHLL and PPYPAWHAPGNI, in complex with the germline mAb 36-65 were determined and compared with the structures of the same Ags bound to another independent germline mAb, BBE6.12H3. For each peptide Ag, the two germline mAbs recognized overlapping epitopes, but in different topologies. The peptide structures differed, and the two paratopes attained discrete conformations, leading to different surface topologies, in a mode that can be described as adjustable locks and flexible keys. This is in contrast to mature mAbs, in which conformational convergence of different paratopes while binding to a common epitope in a similar conformation has been reported. These results suggest that the primary immune receptor repertoire is highly versatile as compared with its mature counterpart. Germline and mature mAbs adopt distinct mechanisms for recognizing a flexible epitope. Whereas conservation of conformational repertoire is a key characteristic of mature mAbs achieved through affinity maturation, the germline mAbs, at the initial stages of Ag encounter, maintain substantial plasticity, accommodating a broad specificity repertoire.

PubMed: 24790145
DOI: 10.4049/JIMMUNOL.1302143

実験手法

X-RAY DIFFRACTION (2.4 Å)