4B9M

Structure of the high fidelity DNA polymerase I with an oxidative formamidopyrimidine-dA DNA lesion -thymine basepair in the post- insertion site.

4B9M の概要

• エントリーDOI: 10.2210/pdb4b9m/pdb
• 関連するPDBエントリー: 2XO7 2XY5 2XY6 2XY7 2Y1I 2Y1J 4B9L 4B9N 4B9S 4B9T 4B9U 4B9V
• 分子名称: DNA POLYMERASE I, 5'-D(*GP*CP*CP*TP*GP*AP*CP*TP*CP*TP*TP)-3', 5'-D(*DC*DA*DA*FAX*AP*GP*AP*GP*TP*CP*AP*GP*GP*CP*TP)-3', ... (6 entities in total)
• 機能のキーワード: transferase-dna complex, oxidative dna lesion, dna damage, translesion dna synthesis, replication, transferase/dna
• 由来する生物種: GEOBACILLUS STEAROTHERMOPHILUS; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 78990.51

構造登録者

Gehrke, T.H.,Lischke, U.,Arnold, S.,Schneider, S.,Carell, T. (登録日: 2012-09-05, 公開日: 2013-04-17, 最終更新日: 2023-12-20)

主引用文献

Gehrke, T.H.,Lischke, U.,Gasteiger, K.L.,Schneider, S.,Arnold, S.,Muller, H.C.,Stephenson, D.S.,Zipse, H.,Carell, T.
Unexpected Non-Hoogsteen-Based Mutagenicity Mechanism of Fapy-DNA Lesions.
Nat.Chem.Biol., 9:455-, 2013

PubMed Abstract: 8-Oxopurines (8-oxodG and 8-oxodA) and formamidopyrimidines (FaPydG and FaPydA) are major oxidative DNA lesions involved in cancer development and aging. Their mutagenicity is believed to result from a conformational shift of the N9-C1' glycosidic bonds from anti to syn, which allows the lesions to form noncanonical Hoogsteen-type base pairs with incoming triphosphates during DNA replication. Here we present biochemical data and what are to our knowledge the first crystal structures of carbocyclic FaPydA and FaPydG containing DNA in complex with a high-fidelity polymerase. Crystallographic snapshots show that the cFaPy lesions keep the anti geometry of the glycosidic bond during error-free and error-prone replication. The observed dG·dC→dT·dA transversion mutations are the result of base shifting and tautomerization.

PubMed: 23685671
DOI: 10.1038/NCHEMBIO.1254

実験手法

X-RAY DIFFRACTION (2.05 Å)