4B93

Complex of Vamp7 cytoplasmic domain with 2nd ankyrin repeat domain of Varp

4B93 の概要

• エントリーDOI: 10.2210/pdb4b93/pdb
• 関連するPDBエントリー: 2VX8 4AFI
• 分子名称: VESICLE-ASSOCIATED MEMBRANE PROTEIN 7, ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27 (3 entities in total)
• 機能のキーワード: endocytosis, exocytosis, snare
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51013.26

構造登録者

Schaefer, I.B.,Owen, D.J.,Luzio, J.P.,Evans, P.R. (登録日: 2012-08-31, 公開日: 2012-10-31, 最終更新日: 2024-05-08)

主引用文献

Schafer, I.B.,Hesketh, G.G.,Bright, N.A.,Gray, S.R.,Pryor, P.R.,Evans, P.R.,Luzio, J.P.,Owen, D.J.
The Binding of Varp to Vamp7 Traps Vamp7 in a Closed, Fusogenically Inactive Conformation.
Nat.Struct.Mol.Biol., 19:1300-, 2012

PubMed Abstract: SNAREs provide energy and specificity to membrane fusion events. Fusogenic trans-SNARE complexes are assembled from glutamine-contributing SNAREs (Q-SNAREs) embedded in one membrane and an arginine-contributing SNARE (R-SNARE) embedded in the other. Regulation of membrane fusion events is crucial for intracellular trafficking. We identify the endosomal protein Varp as an R-SNARE-binding regulator of SNARE complex formation. Varp colocalizes with and binds to VAMP7, an R-SNARE that is involved in both endocytic and secretory pathways. We present the structure of the second ankyrin repeat domain of mammalian Varp in complex with the cytosolic portion of VAMP7. The VAMP7-SNARE motif is trapped between Varp and the VAMP7 longin domain, and hence Varp kinetically inhibits the ability of VAMP7 to form SNARE complexes. This inhibition will be increased when Varp can also bind to other proteins present on the same membrane as VAMP7, such as Rab32-GTP.

PubMed: 23104059
DOI: 10.1038/NSMB.2414

実験手法

X-RAY DIFFRACTION (2 Å)