4B8S

Crystal Structure of Thermococcus litoralis ADP-dependent Glucokinase (GK)

4B8S の概要

• エントリーDOI: 10.2210/pdb4b8s/pdb
• 関連するPDBエントリー: 4B8R
• 分子名称: ADP-DEPENDENT GLUCOKINASE, alpha-D-glucopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: transferase, ribokinase superfamily
• 由来する生物種: THERMOCOCCUS LITORALIS
• 細胞内の位置: Cytoplasm (By similarity): Q7M537
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54676.03

構造登録者

Herrera-Morande, A.,Rivas-Pardo, J.A.,Fernandez, F.J.,Guixe, V.,Vega, M.C. (登録日: 2012-08-30, 公開日: 2013-07-10, 最終更新日: 2023-12-20)

主引用文献

Rivas-Pardo, J.A.,Herrera-Morande, A.,Castro-Fernandez, V.,Fernandez, F.J.,Vega, M.C.,Guixe, V.
Crystal Structure, Saxs and Kinetic Mechanism of Hyperthermophilic Adp-Dependent Glucokinase from Thermococcus Litoralis Reveal a Conserved Mechanism for Catalysis.
Plos One, 8:66687-, 2013

PubMed Abstract: ADP-dependent glucokinases represent a unique family of kinases that belong to the ribokinase superfamily, being present mainly in hyperthermophilic archaea. For these enzymes there is no agreement about the magnitude of the structural transitions associated with ligand binding and whether they are meaningful to the function of the enzyme. We used the ADP-dependent glucokinase from Thermococcus litoralis as a model to investigate the conformational changes observed in X-ray crystallographic structures upon substrate binding and to compare them with those determined in solution in order to understand their interplay with the glucokinase function. Initial velocity studies indicate that catalysis follows a sequential ordered mechanism that correlates with the structural transitions experienced by the enzyme in solution and in the crystal state. The combined data allowed us to resolve the open-closed conformational transition that accounts for the complete reaction cycle and to identify the corresponding clusters of aminoacids residues responsible for it. These results provide molecular bases for a general mechanism conserved across the ADP-dependent kinase family.

PubMed: 23818958
DOI: 10.1371/JOURNAL.PONE.0066687

実験手法

X-RAY DIFFRACTION (2.58 Å)