4B60

Structure of rFnBPA(189-505) in complex with fibrinogen gamma chain C- terminal peptide

Summary for 4B60

• Entry DOI: 10.2210/pdb4b60/pdb
• Related: 4B5Z
• Descriptor: FIBRONECTIN-BINDING PROTEIN A, FIBRINOGEN GAMMA CHAIN, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: cell adhesion, fnbpa, fibrinogen, fibrinogen binding
• Biological source: STAPHYLOCOCCUS AUREUS SUBSP. AUREUS NCTC 8325; More
• Cellular location: Secreted, cell wall; Peptidoglycan-anchor (Potential): P14738; Secreted: P02679
• Total number of polymer chains: 4
• Total formula weight: 74934.29

Authors

Stemberk, V.,Moroz, O.,Atkin, K.E.,Turkenburg, J.P.,Potts, J.R. (deposition date: 2012-08-08, release date: 2013-10-09, Last modification date: 2023-12-20)

Primary citation

Stemberk, V.,Jones, R.P.,Moroz, O.,Atkin, K.E.,Edwards, A.M.,Turkenburg, J.P.,Leech, A.P.,Massey, R.C.,Potts, J.R.
Evidence for Steric Regulation of Fibrinogen Binding to Staphylococcus Aureus Fibronectin-Binding Protein a (Fnbpa).
J.Biol.Chem., 289:12842-, 2014

PubMed Abstract: The adjacent fibrinogen (Fg)- and fibronectin (Fn)-binding sites on Fn-binding protein A (FnBPA), a cell surface protein from Staphylococcus aureus, are implicated in the initiation and persistence of infection. FnBPA contains a single Fg-binding site (that also binds elastin) and multiple Fn-binding sites. Here, we solved the structure of the N2N3 domains containing the Fg-binding site of FnBPA in the apo form and in complex with a Fg peptide. The Fg binding mechanism is similar to that of homologous bacterial proteins but without the requirement for "latch" strand residues. We show that the Fg-binding sites and the most N-terminal Fn-binding sites are nonoverlapping but in close proximity. Although Fg and a subdomain of Fn can form a ternary complex on an FnBPA protein construct containing a Fg-binding site and single Fn-binding site, binding of intact Fn appears to inhibit Fg binding, suggesting steric regulation. Given the concentrations of Fn and Fg in the plasma, this mechanism might result in targeting of S. aureus to fibrin-rich thrombi or elastin-rich tissues.

PubMed: 24627488
DOI: 10.1074/JBC.M113.543546

Experimental method

X-RAY DIFFRACTION (1.83 Å)