4AYB

RNAP at 3.2Ang

4AYB の概要

• エントリーDOI: 10.2210/pdb4ayb/pdb
• 関連するPDBエントリー: 2Y0S 4B1O 4B1P
• 分子名称: DNA-DIRECTED RNA POLYMERASE, ZINC ION, MAGNESIUM ION, ... (16 entities in total)
• 機能のキーワード: transferase, multi-subunit, transcription
• 由来する生物種: SULFOLOBUS SHIBATAE (ARCHAEA); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 406665.54

構造登録者

Wojtas, M.N.,Mogni, M.,Millet, O.,Bell, S.D.,Abrescia, N.G.A. (登録日: 2012-06-19, 公開日: 2012-08-08, 最終更新日: 2024-10-23)

主引用文献

Wojtas, M.N.,Mogni, M.,Millet, O.,Bell, S.D.,Abrescia, N.G.A.
Structural and Functional Analyses of the Interaction of Archaeal RNA Polymerase with DNA.
Nucleic Acids Res., 40:9941-, 2012

PubMed Abstract: Multi-subunit RNA polymerases (RNAPs) in all three domains of life share a common ancestry. The composition of the archaeal RNAP (aRNAP) is not identical between phyla and species, with subunits Rpo8 and Rpo13 found in restricted subsets of archaea. While Rpo8 has an ortholog, Rpb8, in the nuclear eukaryal RNAPs, Rpo13 lacks clear eukaryal orthologs. Here, we report crystal structures of the DNA-bound and free form of the aRNAP from Sulfolobus shibatae. Together with biochemical and biophysical analyses, these data show that Rpo13 C-terminus binds non-specifically to double-stranded DNA. These interactions map on our RNAP-DNA binary complex on the downstream DNA at the far end of the DNA entry channel. Our findings thus support Rpo13 as a RNAP-DNA stabilization factor, a role reminiscent of eukaryotic general transcriptional factors. The data further yield insight into the mechanisms and evolution of RNAP-DNA interaction.

PubMed: 22848102
DOI: 10.1093/NAR/GKS692

実験手法

X-RAY DIFFRACTION (3.202 Å)