4ARV

Yersinia kristensenii phytase apo form

Summary for 4ARV

• Entry DOI: 10.2210/pdb4arv/pdb
• Related: 4ARO 4ARS 4ARU
• Descriptor: PHYTASE, PHOSPHATE ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• Functional Keywords: hydrolase, 6-phytase, myo-inositol hexakis phosphate phosphohydrolase, histidine acid phosphatase, happ
• Biological source: YERSINIA KRISTENSENII
• Total number of polymer chains: 2
• Total formula weight: 98411.66

Authors

Ariza, A.,Moroz, O.V.,Blagova, E.B.,Turkenburg, J.P.,Vevodova, J.,Roberts, S.,Vind, J.,Sjoholm, C.,Lassen, S.F.,De Maria, L.,Glitsoe, V.,Skov, L.K.,Wilson, K.S. (deposition date: 2012-04-26, release date: 2013-05-08, Last modification date: 2024-11-20)

Primary citation

Ariza, A.,Moroz, O.V.,Blagova, E.V.,Turkenburg, J.P.,Waterman, J.,Roberts, S.M.,Vind, J.,Sjoholm, C.,Lassen, S.F.,De Maria, L.,Glitsoe, V.,Skov, L.K.,Wilson, K.S.
Degradation of Phytate by the 6-Phytase from Hafnia Alvei: A Combined Structural and Solution Study.
Plos One, 8:65062-, 2013

PubMed Abstract: Phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. They are used extensively in the feed industry, and have been characterised biochemically and structurally with a number of structures in the PDB. They are divided into four distinct families: histidine acid phosphatases (HAP), β-propeller phytases, cysteine phosphatases and purple acid phosphatases and also split into three enzyme classes, the 3-, 5- and 6-phytases, depending on the position of the first phosphate in the inositol ring to be removed. We report identification, cloning, purification and 3D structures of 6-phytases from two bacteria, Hafnia alvei and Yersinia kristensenii, together with their pH optima, thermal stability, and degradation profiles for phytate. An important result is the structure of the H. alvei enzyme in complex with the substrate analogue myo-inositol hexakissulphate. In contrast to the only previous structure of a ligand-bound 6-phytase, where the 3-phosphate was unexpectedly in the catalytic site, in the H. alvei complex the expected scissile 6-phosphate (sulphate in the inhibitor) is placed in the catalytic site.

PubMed: 23741456
DOI: 10.1371/JOURNAL.PONE.0065062

Experimental method

X-RAY DIFFRACTION (1.67 Å)