4AM3

Crystal structure of C. crescentus PNPase bound to RNA

Summary for 4AM3

Related4AID 4AIM
DescriptorPOLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE, RNA, 5'-R(*UP*AP*AP*CP*UP*UP*UP*GP*GP)-3', PHOSPHATE ION, ... (4 entities in total)
Functional Keywordstransferase-rna complex, kh domain, rnase e, transferase/rna
Biological sourceCAULOBACTER VIBRIOIDES
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Cellular locationCytoplasm (By similarity) Q9AC32
Total number of polymer chains7
Total molecular weight242761.07
Authors
Hardwick, S.W.,Gubbey, T.,Hug, I.,Jenal, U.,Luisi, B.F. (deposition date: 2012-03-07, release date: 2012-04-18, Last modification date: 2014-02-05)
Primary citation
Hardwick, S.W.,Gubbey, T.,Hug, I.,Jenal, U.,Luisi, B.F.
Crystal Structure of Caulobacter Crescentus Polynucleotide Phosphorylase Reveals a Mechanism of RNA Substrate Channelling and RNA Degradosome Assembly.
Open Biol., 2:20028-, 2012
PubMed: 22724061 (PDB entries with the same primary citation)
DOI: 10.1098/RSOB.120028
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.253225.0%3.6%1.3%0.02MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution