4ALZ

The Yersinia T3SS basal body component YscD reveals a different structural periplasmatic domain organization to known homologue PrgH

4ALZ の概要

• エントリーDOI: 10.2210/pdb4alz/pdb
• 分子名称: YOP PROTEINS TRANSLOCATION PROTEIN D, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: membrane protein, t3ss, type 3 secretion, effector transport
• 由来する生物種: YERSINIA ENTEROCOLITICA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22741.51

構造登録者

Schmelz, S.,Wisand, U.,Stenta, M.,Muenich, S.,Widow, U.,Cornelis, G.R.,Heinz, D.W. (登録日: 2012-03-06, 公開日: 2013-04-24, 最終更新日: 2024-05-08)

主引用文献

Kudryashev, M.,Stenta, M.,Schmelz, S.,Amstutz, M.,Wiesand, U.,Castano-Diez, D.,Degiacomi, M.T.,Munnich, S.,Bleck, C.K.,Kowal, J.,Diepold, A.,Heinz, D.W.,Dal Peraro, M.,Cornelis, G.R.,Stahlberg, H.
In Situ Structural Analysis of the Yersinia Enterocolitica Injectisome.
Elife, 2:00792-, 2013

PubMed Abstract: Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica and Shigella flexneri injectisomes in situ and the first structural analysis of the Yersinia injectisome. Unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had similar dimensions and were significantly longer than the isolated structures of related injectisomes. The crystal structure of the inner membrane injectisome component YscD appeared elongated compared to a homologous protein, and molecular dynamics simulations documented its elongation elasticity. The ring-shaped secretin YscC at the outer membrane was stretched by 30-40% in situ, compared to its isolated liposome-embedded conformation. We suggest that elasticity is critical for some two-membrane spanning protein complexes to cope with variations in the intermembrane distance. DOI:http://dx.doi.org/10.7554/eLife.00792.001.

PubMed: 23908767
DOI: 10.7554/ELIFE.00792

実験手法

X-RAY DIFFRACTION (1.4 Å)