4AJ5

Crystal structure of the Ska core complex

4AJ5 の概要

• エントリーDOI: 10.2210/pdb4aj5/pdb
• 分子名称: SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 3, SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1, SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2 (3 entities in total)
• 機能のキーワード: cell cycle, ska complex, mitosis, cell division, kinetochore-microtubule attachment, kmn network, ndc80 complex, dam1/dash
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton, spindle: Q8IX90 Q96BD8 Q8WVK7
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 367169.58

構造登録者

Jeyaprakash, A.A.,Santamaria, A.,Jayachandran, U.,Chan, Y.W.,Benda, C.,Nigg, E.A.,Conti, E. (登録日: 2012-02-15, 公開日: 2012-05-23)

主引用文献

Jeyaprakash, A.A.,Santamaria, A.,Jayachandran, U.,Chan, Y.W.,Benda, C.,Nigg, E.A.,Conti, E.
Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface.
Mol.Cell, 46:274-, 2012

PubMed Abstract: The Ska complex is an essential mitotic component required for accurate cell division in human cells. It is composed of three subunits that function together to establish stable kinetochore-microtubule interactions in concert with the Ndc80 network. We show that the structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3. The C-terminal domains of Ska1 and Ska3 protrude at each end of the homodimer, bind microtubules in vitro when connected to the central core, and are essential in vivo. Mutations disrupting the central coiled coil or the dimerization interface result in chromosome congression failure followed by cell death. The Ska complex is thus endowed with bipartite and cooperative tubulin-binding properties at the ends of a 350 Å-long molecule. We discuss how this symmetric architecture might complement and stabilize the Ndc80-microtubule attachments with analogies to the yeast Dam1/DASH complex.

PubMed: 22483620
DOI: 10.1016/J.MOLCEL.2012.03.005

実験手法

X-RAY DIFFRACTION (3.32 Å)