4ADZ

Crystal Structure of the apo form of a Copper-sensitive operon Regulator (CsoR) protein from Streptomyces lividans

Summary for 4ADZ

• Entry DOI: 10.2210/pdb4adz/pdb
• Descriptor: CSOR, SULFATE ION (3 entities in total)
• Functional Keywords: transcription, copper sensor
• Biological source: STREPTOMYCES LIVIDANS
• Total number of polymer chains: 2
• Total formula weight: 30101.52

Authors

Dwarakanath, S.,Hough, M.A.,Worrall, J.A.R. (deposition date: 2012-01-04, release date: 2012-04-25, Last modification date: 2024-05-08)

Primary citation

Dwarakanath, S.,Chaplin, A.K.,Hough, M.A.,Rigali, S.,Vijgenboom, E.,Worrall, J.A.R.
The Response to Copper Stress in Streptomyces Lividans Extends Beyond Genes Under the Direct Control of a Copper Sensitive Operon Repressor Protein (Csor)
J.Biol.Chem., 287:17833-, 2012

PubMed Abstract: A copper-sensitive operon repressor protein (CsoR) has been identified in Streptomyces lividans (CsoR(Sl)) and found to regulate copper homeostasis with attomolar affinity for Cu(I). Solution studies reveal apo- and Cu(I)-CsoR(Sl) to be a tetramer assembly, and a 1.7-Å resolution crystal structure of apo-CsoR(Sl) reveals that a significant conformational change is necessary to enable Cu(I) binding. In silico prediction of the CsoR regulon was confirmed in vitro (EMSA) and in vivo (RNA-seq), which highlighted that next to the csoR gene itself, the regulon consists of two Cu(I) efflux systems involving a CopZ-like copper metallochaperone protein and a CopA P(1)-type ATPase. Although deletion of csoR has only minor effects on S. lividans development when grown under high copper concentrations, mutations of the Cu(I) ligands decrease tolerance to copper as a result of the Cu(I)-CsoR mutants failing to disengage from the DNA targets, thus inhibiting the derepression of the regulon. RNA-seq experiments carried out on samples incubated with exogenous copper and a ΔcsoR strain showed that the set of genes responding to copper stress is much wider than anticipated and largely extends beyond genes targeted by CsoR. This suggests more control levels are operating and directing other regulons in copper homeostasis beside the CsoR regulon.

PubMed: 22451651
DOI: 10.1074/JBC.M112.352740

Experimental method

X-RAY DIFFRACTION (1.7 Å)