4ADZ

Crystal Structure of the apo form of a Copper-sensitive operon Regulator (CsoR) protein from Streptomyces lividans

4ADZ の概要

• エントリーDOI: 10.2210/pdb4adz/pdb
• 分子名称: CSOR, SULFATE ION (3 entities in total)
• 機能のキーワード: transcription, copper sensor
• 由来する生物種: STREPTOMYCES LIVIDANS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30101.52

構造登録者

Dwarakanath, S.,Hough, M.A.,Worrall, J.A.R. (登録日: 2012-01-04, 公開日: 2012-04-25, 最終更新日: 2024-05-08)

主引用文献

Dwarakanath, S.,Chaplin, A.K.,Hough, M.A.,Rigali, S.,Vijgenboom, E.,Worrall, J.A.R.
The Response to Copper Stress in Streptomyces Lividans Extends Beyond Genes Under the Direct Control of a Copper Sensitive Operon Repressor Protein (Csor)
J.Biol.Chem., 287:17833-, 2012

PubMed Abstract: A copper-sensitive operon repressor protein (CsoR) has been identified in Streptomyces lividans (CsoR(Sl)) and found to regulate copper homeostasis with attomolar affinity for Cu(I). Solution studies reveal apo- and Cu(I)-CsoR(Sl) to be a tetramer assembly, and a 1.7-Å resolution crystal structure of apo-CsoR(Sl) reveals that a significant conformational change is necessary to enable Cu(I) binding. In silico prediction of the CsoR regulon was confirmed in vitro (EMSA) and in vivo (RNA-seq), which highlighted that next to the csoR gene itself, the regulon consists of two Cu(I) efflux systems involving a CopZ-like copper metallochaperone protein and a CopA P(1)-type ATPase. Although deletion of csoR has only minor effects on S. lividans development when grown under high copper concentrations, mutations of the Cu(I) ligands decrease tolerance to copper as a result of the Cu(I)-CsoR mutants failing to disengage from the DNA targets, thus inhibiting the derepression of the regulon. RNA-seq experiments carried out on samples incubated with exogenous copper and a ΔcsoR strain showed that the set of genes responding to copper stress is much wider than anticipated and largely extends beyond genes targeted by CsoR. This suggests more control levels are operating and directing other regulons in copper homeostasis beside the CsoR regulon.

PubMed: 22451651
DOI: 10.1074/JBC.M112.352740

実験手法

X-RAY DIFFRACTION (1.7 Å)