4ACO

Structure of the budding yeast Ndc10 N-terminal domain

4ACO の概要

• エントリーDOI: 10.2210/pdb4aco/pdb
• 分子名称: CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT A (2 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: SACCHAROMYCES CEREVISIAE
• 細胞内の位置: Nucleus: P32504
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 112066.03

構造登録者

Perriches, T.,Singleton, M.R. (登録日: 2011-12-16, 公開日: 2012-01-11, 最終更新日: 2024-05-08)

主引用文献

Perriches, T.,Singleton, M.R.
The Structure of the Yeast Kinetochore Ndc10 DNA-Binding Domain Reveals an Unexpected Evolutionary Relationship to Tyrosine Recombinases.
J.Biol.Chem., 287:5173-, 2012

PubMed Abstract: We have solved the x-ray structure of the N-terminal half of the yeast kinetochore protein Ndc10 at 1.9 Å resolution. This essential protein is a key constituent of the budding yeast centromere and is essential for the recruitment of the centromeric nucleosome and establishment of the kinetochore. The fold of the protein shows unexpected similarities to the tyrosine recombinase/λ-integrase family of proteins, most notably Cre, with some variation in the relative position of the subdomains. This finding offers new insights into kinetochore evolution and the adaptation of a well studied protein fold to a novel role. By comparison with tyrosine recombinases and mutagenesis studies, we have been able to define some of the key DNA-binding motifs.

PubMed: 22215672
DOI: 10.1074/JBC.C111.318501

実験手法

X-RAY DIFFRACTION (1.89 Å)