4A82

Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.

4A82 の概要

• エントリーDOI: 10.2210/pdb4a82/pdb
• EMDBエントリー: 1966
• 分子名称: CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (1 entity in total)
• 機能のキーワード: cystic fibrosis, cftr, ion channel, transport protein, cassette protein
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 259735.86

構造登録者

Rosenberg, M.F.,ORyan, L.P.,Hughes, G.,Zhao, Z.,Aleksandrov, L.A.,Riordan, J.R.,Ford, R.C. (登録日: 2011-11-18, 公開日: 2012-01-11, 最終更新日: 2024-05-08)

主引用文献

Rosenberg, M.F.,O'Ryan, L.,Hughes, G.,Zhao, Z.,Aleksandrov, L.A.,Riordan, J.R.,Ford, R.C.
The Cystic Fibrosis Transmembrane Conductance Regulator (Cftr):3D Structure and Localisation of a Channel Gate.
J.Biol.Chem., 286:42647-, 2011

PubMed Abstract: Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance regulator (CFTR). We have studied CFTR structure by electron crystallography. The data were compared with existing structures of other ATP-binding cassette transporters. The protein was crystallized in the outward facing state and resembled the well characterized Sav1866 transporter. We identified regions in the CFTR map, not accounted for by Sav1866, which were potential locations for the regulatory region as well as the channel gate. In this analysis, we were aided by the fact that the unit cell was composed of two molecules not related by crystallographic symmetry. We also identified regions in the fitted Sav1866 model that were missing from the map, hence regions that were either disordered in CFTR or differently organized compared with Sav1866. Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located).

PubMed: 21931164
DOI: 10.1074/JBC.M111.292268

実験手法

ELECTRON CRYSTALLOGRAPHY (9 Å)