4A61

ParM from plasmid R1 in complex with AMPPNP

4A61 の概要

• エントリーDOI: 10.2210/pdb4a61/pdb
• 関連するPDBエントリー: 1MWK 1MWM 4A62 4A6J
• 分子名称: PLASMID SEGREGATION PROTEIN PARM, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: transport protein, plasmid segregation, actin-fold
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37207.78

構造登録者

van den Ent, F.,Moller-Jensen, J.,Gayathri, P.,Lowe, J. (登録日: 2011-10-31, 公開日: 2012-11-07, 最終更新日: 2023-12-20)

主引用文献

Gayathri, P.,Fujii, T.,Moller-Jensen, J.,Van Den Ent, F.,Namba, K.,Lowe, J.
A Bipolar Spindle of Antiparallel Parm Filaments Drives Bacterial Plasmid Segregation.
Science, 338:1334-, 2012

PubMed Abstract: To ensure their stable inheritance by daughter cells during cell division, bacterial low-copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between sister plasmids. In the ParMRC system of the Escherichia coli R1 plasmid, ParM, an actinlike protein, forms the spindle between ParRC complexes on sister plasmids. By using a combination of structural work and total internal reflection fluorescence microscopy, we show that ParRC bound and could accelerate growth at only one end of polar ParM filaments, mechanistically resembling eukaryotic formins. The architecture of ParM filaments enabled two ParRC-bound filaments to associate in an antiparallel orientation, forming a bipolar spindle. The spindle elongated as a bundle of at least two antiparallel filaments, thereby pushing two plasmid clusters toward the poles.

PubMed: 23112295
DOI: 10.1126/SCIENCE.1229091

実験手法

X-RAY DIFFRACTION (2 Å)