4A35

Crystal structure of human Mitochondrial enolase superfamily member 1 (ENOSF1)

4A35 の概要

• エントリーDOI: 10.2210/pdb4a35/pdb
• 分子名称: MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1, MAGNESIUM ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: isomerase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50050.23

構造登録者

Muniz, J.R.C.,Froese, D.S.,Krojer, T.,Vollmar, M.,Canning, P.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Weigelt, J.,Bountra, C.,Oppermann, U.,Yue, W.W. (登録日: 2011-09-30, 公開日: 2011-10-12, 最終更新日: 2024-05-08)

主引用文献

Wichelecki, D.J.,Froese, D.S.,Kopec, J.,Muniz, J.R.,Yue, W.W.,Gerlt, J.A.
Enzymatic and structural characterization of rTS gamma provides insights into the function of rTS beta.
Biochemistry, 53:2732-2738, 2014

PubMed Abstract: In humans, the gene encoding a reverse thymidylate synthase (rTS) is transcribed in the reverse direction of the gene encoding thymidylate synthase (TS) that is involved in DNA biosynthesis. Three isoforms are found: α, β, and γ, with the transcript of the α-isoform overlapping with that of TS. rTSβ has been of interest since the discovery of its overexpression in methotrexate and 5-fluorouracil resistant cell lines. Despite more than 20 years of study, none of the rTS isoforms have been biochemically or structurally characterized. In this study, we identified rTSγ as an l-fuconate dehydratase and determined its high-resolution crystal structure. Our data provide an explanation for the observed difference in enzymatic activities between rTSβ and rTSγ, enabling more informed proposals for the possible function of rTSβ in chemotherapeutic resistance.

PubMed: 24697329
DOI: 10.1021/bi500349e

実験手法

X-RAY DIFFRACTION (1.74 Å)