4A11

Structure of the hsDDB1-hsCSA complex

Summary for 4A11

• Entry DOI: 10.2210/pdb4a11/pdb
• Related: 2B5L 2B5M 2B5N 2HYE 3EI1 3EI2 3EI3 3EI4 4A08 4A09 4A0A 4A0B 4A0K 4A0L
• Descriptor: DNA DAMAGE-BINDING PROTEIN 1, DNA EXCISION REPAIR PROTEIN ERCC-8 (2 entities in total)
• Functional Keywords: dna binding protein, dna damage repair
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 2
• Total formula weight: 174860.51

Authors

Bohm, K.,Scrima, A.,Fischer, E.S.,Gut, H.,Thomae, N.H. (deposition date: 2011-09-13, release date: 2011-12-07, Last modification date: 2023-12-20)

Primary citation

Fischer, E.S.,Scrima, A.,Bohm, K.,Matsumoto, S.,Lingaraju, G.M.,Faty, M.,Yasuda, T.,Cavadini, S.,Wakasugi, M.,Hanaoka, F.,Iwai, S.,Gut, H.,Sugasawa, K.,Thoma, N.H.
The Molecular Basis of Crl4(Ddb2/Csa) Ubiquitin Ligase Architecture, Targeting, and Activation.
Cell(Cambridge,Mass.), 147:1024-, 2011

PubMed Abstract: The DDB1-CUL4-RBX1 (CRL4) ubiquitin ligase family regulates a diverse set of cellular pathways through dedicated substrate receptors (DCAFs). The DCAF DDB2 detects UV-induced pyrimidine dimers in the genome and facilitates nucleotide excision repair. We provide the molecular basis for DDB2 receptor-mediated cyclobutane pyrimidine dimer recognition in chromatin. The structures of the fully assembled DDB1-DDB2-CUL4A/B-RBX1 (CRL4(DDB2)) ligases reveal that the mobility of the ligase arm creates a defined ubiquitination zone around the damage, which precludes direct ligase activation by DNA lesions. Instead, the COP9 signalosome (CSN) mediates the CRL4(DDB2) inhibition in a CSN5 independent, nonenzymatic, fashion. In turn, CSN inhibition is relieved upon DNA damage binding to the DDB2 module within CSN-CRL4(DDB2). The Cockayne syndrome A DCAF complex crystal structure shows that CRL4(DCAF(WD40)) ligases share common architectural features. Our data support a general mechanism of ligase activation, which is induced by CSN displacement from CRL4(DCAF) on substrate binding to the DCAF.

PubMed: 22118460
DOI: 10.1016/J.CELL.2011.10.035

Experimental method

X-RAY DIFFRACTION (3.31 Å)