4ZCJ

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA1 Cys30, HA2 Cys47 mutant

Summary for 4ZCJ

• Entry DOI: 10.2210/pdb4zcj/pdb
• Descriptor: Hemagglutinin, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: influenza, hemagglutinin, glycoprotein, viral protein
• Biological source: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); More
• Total number of polymer chains: 6
• Total formula weight: 169160.92

Authors

Lee, P.S.,Wilson, I.A. (deposition date: 2015-04-16, release date: 2015-05-13, Last modification date: 2024-10-30)

Primary citation

Lee, P.S.,Zhu, X.,Yu, W.,Wilson, I.A.
Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.
J.Virol., 89:7417-7420, 2015

PubMed Abstract: We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza virus HA immunogens.

PubMed: 25926650
DOI: 10.1128/JVI.00808-15

Experimental method

X-RAY DIFFRACTION (3 Å)