4ZB2
A native form of glucose isomerase collected at room temperature.
Summary for 4ZB2
| Entry DOI | 10.2210/pdb4zb2/pdb |
| Related | 4ZB5 4ZBC 4ZBO |
| Descriptor | Xylose isomerase, MANGANESE (II) ION, alpha-D-glucopyranose, ... (5 entities in total) |
| Functional Keywords | isomerase |
| Biological source | Streptomyces rubiginosus |
| Cellular location | Cytoplasm: P24300 |
| Total number of polymer chains | 1 |
| Total formula weight | 43753.49 |
| Authors | Sandy, J. (deposition date: 2015-04-14, release date: 2016-03-23, Last modification date: 2024-01-10) |
| Primary citation | Lobley, C.M.,Sandy, J.,Sanchez-Weatherby, J.,Mazzorana, M.,Krojer, T.,Nowak, R.P.,Sorensen, T.L. A generic protocol for protein crystal dehydration using the HC1b humidity controller. Acta Crystallogr D Struct Biol, 72:629-640, 2016 Cited by PubMed Abstract: Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a precise yet hassle-free approach to altering crystal hydration. A protocol is introduced to assess the impact of crystal dehydration systematically applied to nine experimental crystal systems. In one case, that of glucose isomerase, dehydration triggering a change of space group from I222 to P21212 was observed. This observation is supported by an extended study of the behaviour of the glucose isomerase crystal structure during crystal dehydration. PubMed: 27139626DOI: 10.1107/S2059798316003065 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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