4YFG

Crystal structure of PTP delta meA3/meB minus variant Ig1-Fn1

Summary for 4YFG

• Entry DOI: 10.2210/pdb4yfg/pdb
• Related: 4YFC 4YFD 4YFE
• Descriptor: Receptor-type tyrosine-protein phosphatase delta, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: synapse organizer, hydrolase
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 2
• Total formula weight: 108403.33

Authors

Yamagata, A.,Fukai, S. (deposition date: 2015-02-25, release date: 2015-05-06, Last modification date: 2024-10-16)

Primary citation

Yamagata, A.,Yoshida, T.,Sato, Y.,Goto-Ito, S.,Uemura, T.,Maeda, A.,Shiroshima, T.,Iwasawa-Okamoto, S.,Mori, H.,Mishina, M.,Fukai, S.
Mechanisms of splicing-dependent trans-synaptic adhesion by PTP delta-IL1RAPL1/IL-1RAcP for synaptic differentiation.
Nat Commun, 6:6926-6926, 2015

PubMed Abstract: Synapse formation is triggered through trans-synaptic interaction between pairs of pre- and postsynaptic adhesion molecules, the specificity of which depends on splice inserts known as 'splice-insert signaling codes'. Receptor protein tyrosine phosphatase δ (PTPδ) can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to interleukin-1 receptor accessory protein (IL-1RAcP) and IL-1RAcP-like-1 (IL1RAPL1) in a splicing-dependent manner. Here, we report crystal structures of PTPδ in complex with IL1RAPL1 and IL-1RAcP. The first immunoglobulin-like (Ig) domain of IL1RAPL1 directly recognizes the first splice insert, which is critical for binding to IL1RAPL1. The second splice insert functions as an adjustable linker that positions the Ig2 and Ig3 domains of PTPδ for simultaneously interacting with the Ig1 domain of IL1RAPL1 or IL-1RAcP. We further identified the IL1RAPL1-specific interaction, which appears coupled to the first-splice-insert-mediated interaction. Our results thus reveal the decoding mechanism of splice-insert signaling codes for synaptic differentiation induced by trans-synaptic adhesion between PTPδ and IL1RAPL1/IL-1RAcP.

PubMed: 25908590
DOI: 10.1038/ncomms7926

Experimental method

X-RAY DIFFRACTION (3.491 Å)