4UZM
Shotgun proteolysis: A practical application
Summary for 4UZM
| Entry DOI | 10.2210/pdb4uzm/pdb |
| Related | 4UZW 4UZX |
| NMR Information | BMRB: 25210 |
| Descriptor | PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG (1 entity in total) |
| Functional Keywords | structural protein |
| Biological source | ESCHERICHIA COLI |
| Cellular location | Cell inner membrane; Multi-pass membrane protein: P45800 |
| Total number of polymer chains | 1 |
| Total formula weight | 13435.93 |
| Authors | Allen, M.D.,Bycroft, M.,Freund, S.M.V.,Christ, D. (deposition date: 2014-09-05, release date: 2014-09-17, Last modification date: 2024-05-15) |
| Primary citation | Allen, M.D.,Christie, M.,Jones, P.,Porebski, B.T.,Roome, B.,Freund, S.M.,Buckle, A.M.,Bycroft, M.,Christ, D. Solution Structure of a Soluble Fragment Derived from a Membrane Protein by Shotgun Proteolysis. Protein Eng.Des.Sel., 28:445-, 2015 Cited by PubMed Abstract: We have previously reported a phage display method for the identification of protein domains on a genome-wide scale (shotgun proteolysis). Here we present the solution structure of a fragment of the Escherichia coli membrane protein yrfF, as identified by shotgun proteolysis, and determined by NMR spectroscopy. Despite the absence of computational predictions, the fragment formed a well-defined beta-barrel structure, distantly falling within the OB-fold classification. Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies. PubMed: 25877662DOI: 10.1093/PROTEIN/GZV021 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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