4QRT

Crystal Structure of HLA B*0801 in complex with ELN_YYM, ELNRKMIYM

Summary for 4QRT

• Entry DOI: 10.2210/pdb4qrt/pdb
• Related: 4QRP 4QRQ 4QRR 4QRS 4QRT
• Descriptor: HLA class I histocompatibility antigen, B-8 alpha chain, Beta-2-microglobulin, Major immediate-early protein, ... (5 entities in total)
• Functional Keywords: hla b*0801, cmv, tcr, t cell, immune system
• Biological source: Homo sapiens (human); More
• Cellular location: Membrane; Single-pass type I membrane protein: P30460; Secreted: P61769
• Total number of polymer chains: 3
• Total formula weight: 45068.89

Authors

Gras, S.,Twist, K.-A.,Rossjohn, J. (deposition date: 2014-07-02, release date: 2014-07-16, Last modification date: 2024-10-30)

Primary citation

Smith, C.,Gras, S.,Brennan, R.M.,Bird, N.L.,Valkenburg, S.A.,Twist, K.A.,Burrows, J.M.,Miles, J.J.,Chambers, D.,Bell, S.,Campbell, S.,Kedzierska, K.,Burrows, S.R.,Rossjohn, J.,Khanna, R.
Molecular imprint of exposure to naturally occurring genetic variants of human cytomegalovirus on the T cell repertoire.
Sci Rep, 4:3993-3993, 2014

PubMed Abstract: Exposure to naturally occurring variants of herpesviruses in clinical settings can have a dramatic impact on anti-viral immunity. Here we have evaluated the molecular imprint of variant peptide-MHC complexes on the T-cell repertoire during human cytomegalovirus (CMV) infection and demonstrate that primary co-infection with genetic variants of CMV was coincident with development of strain-specific T-cell immunity followed by emergence of cross-reactive virus-specific T-cells. Cross-reactive CMV-specific T cells exhibited a highly conserved public T cell repertoire, while T cells directed towards specific genetic variants displayed oligoclonal repertoires, unique to each individual. T cell recognition foot-print and pMHC-I structural analyses revealed that the cross-reactive T cells accommodate alterations in the pMHC complex with a broader foot-print focussing on the core of the peptide epitope. These findings provide novel molecular insight into how infection with naturally occurring genetic variants of persistent human herpesviruses imprints on the evolution of the anti-viral T-cell repertoire.

PubMed: 24509977
DOI: 10.1038/srep03993

Experimental method

X-RAY DIFFRACTION (1.4 Å)