4N9B

Fragment-based Design of 3-Aminopyridine-derived Amides as Potent Inhibitors of Human Nicotinamide Phosphoribosyltransferase (NAMPT)

Summary for 4N9B

• Entry DOI: 10.2210/pdb4n9b/pdb
• Related: 4N9C 4N9D 4N9E
• Descriptor: Nicotinamide phosphoribosyltransferase, PHOSPHATE ION, 1-methyl-N-(pyridin-3-yl)-1H-pyrazole-5-carboxamide, ... (4 entities in total)
• Functional Keywords: transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 114479.12

Authors

Dragovich, P.S.,Zhao, G.,Baumeister, T.,Bravo, B.,Giannetti, A.M.,Ho, Y.,Hua, R.,Li, G.,Liang, X.,O'Brien, T.,Skelton, N.J.,Wang, C.,Zhai, Q.,Oh, A.,Wang, W.,Wang, Y.,Xiao, Y.,Yuen, P.,Zak, M.,Zheng, X. (deposition date: 2013-10-20, release date: 2014-02-19, Last modification date: 2023-09-20)

Primary citation

Dragovich, P.S.,Zhao, G.,Baumeister, T.,Bravo, B.,Giannetti, A.M.,Ho, Y.C.,Hua, R.,Li, G.,Liang, X.,Ma, X.,O'Brien, T.,Oh, A.,Skelton, N.J.,Wang, C.,Wang, W.,Wang, Y.,Xiao, Y.,Yuen, P.W.,Zak, M.,Zhao, Q.,Zheng, X.
Fragment-based design of 3-aminopyridine-derived amides as potent inhibitors of human nicotinamide phosphoribosyltransferase (NAMPT).
Bioorg.Med.Chem.Lett., 24:954-962, 2014

PubMed Abstract: The fragment-based identification of two novel and potent biochemical inhibitors of the nicotinamide phosphoribosyltransferase (NAMPT) enzyme is described. These compounds (51 and 63) incorporate an amide moiety derived from 3-aminopyridine, and are thus structurally distinct from other known anti-NAMPT agents. Each exhibits potent inhibition of NAMPT biochemical activity (IC50=19 and 15 nM, respectively) as well as robust antiproliferative properties in A2780 cell culture experiments (IC50=121 and 99 nM, respectively). However, additional biological studies indicate that only inhibitor 51 exerts its A2780 cell culture effects via a NAMPT-mediated mechanism. The crystal structures of both 51 and 63 in complex with NAMPT are also independently described.

PubMed: 24433859
DOI: 10.1016/j.bmcl.2013.12.062

Experimental method

X-RAY DIFFRACTION (2.859 Å)