4M45
RB69 DNA polymerase ternary complex with dG/dT at position n-5 of primer/template duplex
Summary for 4M45
| Entry DOI | 10.2210/pdb4m45/pdb |
| Related | 4M3R 4M3T 4M3U 4M3W 4M3X 4M3Y 4M3Z 4M41 4M42 |
| Descriptor | DNA polymerase, DNA template, DNA primer, ... (6 entities in total) |
| Functional Keywords | rb69 pol, quadruple, dg/dt, n-5, rb69, hydrolase-dna complex, hydrolase/dna |
| Biological source | Enterobacteria phage RB69 More |
| Total number of polymer chains | 3 |
| Total formula weight | 114078.24 |
| Authors | Xia, S.,Konigsberg, W.H. (deposition date: 2013-08-06, release date: 2014-02-12, Last modification date: 2024-02-28) |
| Primary citation | Xia, S.,Konigsberg, W.H. Mispairs with Watson-Crick base-pair geometry observed in ternary complexes of an RB69 DNA polymerase variant. Protein Sci., 23:508-513, 2014 Cited by PubMed Abstract: Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson-Crick geometry in the presence of Mn(2+) indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base-pair could be affected by its microenvironment, we determined 10 structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n-1 to n-5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson-Crick geometry, have been observed, strongly suggesting that the local environment of base-pairs plays an important role in their tautomeric or ionization states. PubMed: 24458997DOI: 10.1002/pro.2434 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.89 Å) |
Structure validation
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