4EI5

Crystal Structure of XV19 TCR in complex with CD1d-sulfatide C24:1

Summary for 4EI5

• Entry DOI: 10.2210/pdb4ei5/pdb
• Related: 2AKR 4EI6
• Descriptor: Antigen-presenting glycoprotein CD1d1, (15Z)-N-((1S,2R,3E)-2-HYDROXY-1-{[(3-O-SULFO-BETA-D-GALACTOPYRANOSYL)OXY]METHYL}HEPTADEC-3-ENYL)TETRACOS-15-ENAMIDE, Beta-2-microglobulin, ... (10 entities in total)
• Functional Keywords: sulfatide, lipid, cd1d, nkt typeii, tcr, immune system
• Biological source: Mus musculus (mouse); More
• Total number of polymer chains: 8
• Total formula weight: 199821.14

Authors

Patel, O.,Gras, S.,Rossjohn, J. (deposition date: 2012-04-04, release date: 2012-07-25, Last modification date: 2023-09-13)

Primary citation

Patel, O.,Pellicci, D.G.,Gras, S.,Sandoval-Romero, M.L.,Uldrich, A.P.,Mallevaey, T.,Clarke, A.J.,Le Nours, J.,Theodossis, A.,Cardell, S.L.,Gapin, L.,Godfrey, D.I.,Rossjohn, J.
Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor.
Nat.Immunol., 13:857-863, 2012

PubMed Abstract: Natural killer T cells (NKT cells) are divided into type I and type II subsets on the basis of differences in their T cell antigen receptor (TCR) repertoire and CD1d-antigen specificity. Although the mode by which type I NKT cell TCRs recognize CD1d-antigen has been established, how type II NKT cell TCRs engage CD1d-antigen is unknown. Here we provide a basis for how a type II NKT cell TCR, XV19, recognized CD1d-sulfatide. The XV19 TCR bound orthogonally above the A' pocket of CD1d, in contrast to the parallel docking of type I NKT cell TCRs over the F' pocket of CD1d. At the XV19 TCR-CD1d-sulfatide interface, the TCRα and TCRβ chains sat centrally on CD1d, where the malleable CDR3 loops dominated interactions with CD1d-sulfatide. Accordingly, we highlight the diverse mechanisms by which NKT cell TCRs can bind CD1d and account for the distinct antigen specificity of type II NKT cells.

PubMed: 22820603
DOI: 10.1038/ni.2372

Experimental method

X-RAY DIFFRACTION (3.1 Å)