4E5X

Crystal structure of a complex between the human adenovirus type 2 E3-19K protein and MHC class I molecule HLA-A2/Tax

Summary for 4E5X

• Entry DOI: 10.2210/pdb4e5x/pdb
• Descriptor: HLA class I histocompatibility antigen, A-2 alpha chain, Beta-2-microglobulin, Protein Tax-1, ... (5 entities in total)
• Functional Keywords: ad2 e3-19k-hla-a2 complex, unique tertiary structure, adenovirus e3-19k, immune evasion function, mhc class i molecule, endoplasmic reticulum, immune system-transcription complex, immune system/transcription
• Biological source: Homo sapiens (human); More
• Cellular location: Membrane; Single-pass type I membrane protein: P01892; Secreted: P61769; Host nucleus (By similarity): P0C213; Host endoplasmic reticulum membrane; Single- pass type I membrane protein: P68979
• Total number of polymer chains: 8
• Total formula weight: 113485.32

Authors

Li, L.,Bouvier, M. (deposition date: 2012-03-14, release date: 2012-10-10, Last modification date: 2024-11-27)

Primary citation

Li, L.,Muzahim, Y.,Bouvier, M.
Crystal structure of adenovirus E3-19K bound to HLA-A2 reveals mechanism for immunomodulation.
Nat.Struct.Mol.Biol., 19:1176-1181, 2012

PubMed Abstract: E3-19K binds to and retains MHC class I molecules in the endoplasmic reticulum, suppressing anti-adenovirus activities of T cells. We determined the structure of the adenovirus serotype 2 (Ad2, species C) E3-19K-HLA-A2 complex to 1.95-Å resolution. Ad2 E3-19K binds to the N terminus of the HLA-A2 groove, contacting the α1, α2 and α3 domains and β(2)m. Ad2 E3-19K has a unique structure comprising a large N-terminal domain, formed by two partially overlapping β-sheets arranged in a V shape, and a C-terminal α-helix and tail. The structure reveals determinants in E3-19K and HLA-A2 that are important for complex formation; conservation of some of these determinants in E3-19K proteins of different species and MHC I molecules of different loci suggests a universal binding mode for all E3-19K proteins. Our structure is important for understanding the immunomodulatory function of E3-19K.

PubMed: 23042604
DOI: 10.1038/nsmb.2396

Experimental method

X-RAY DIFFRACTION (1.95 Å)