4UJE
Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
This is a non-PDB format compatible entry.
Summary for 4UJE
| Entry DOI | 10.2210/pdb4uje/pdb |
| Related | 4CXG 4CXH |
| EMDB information | 2620 |
| Descriptor | TRNA-LYS, 40S RIBOSOMAL PROTEIN S5, 40S RIBOSOMAL PROTEIN S6, ... (83 entities in total) |
| Functional Keywords | ribosome, translation, mammalian 80s ribosome, elongation cycle, post-translocational state, trna selection |
| Biological source | ESCHERICHIA COLI More |
| Total number of polymer chains | 83 |
| Total formula weight | 3858796.30 |
| Authors | Budkevich, T.V.,Giesebrecht, J.,Behrmann, E.,Loerke, J.,Ramrath, D.J.F.,Mielke, T.,Ismer, J.,Hildebrand, P.,Tung, C.-S.,Nierhaus, K.H.,Sanbonmatsu, K.Y.,Spahn, C.M.T. (deposition date: 2014-04-05, release date: 2014-07-16, Last modification date: 2024-11-13) |
| Primary citation | Budkevich, T.V.,Giesebrecht, J.,Behrmann, E.,Loerke, J.,Ramrath, D.J.,Mielke, T.,Ismer, J.,Hildebrand, P.W.,Tung, C.S.,Nierhaus, K.H.,Sanbonmatsu, K.Y.,Spahn, C.M. Regulation of the Mammalian Elongation Cycle by Subunit Rolling: A Eukaryotic-Specific Ribosome Rearrangement. Cell(Cambridge,Mass.), 158:121-, 2014 Cited by PubMed Abstract: The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1A⋅Val-tRNA⋅GMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection. PubMed: 24995983DOI: 10.1016/J.CELL.2014.04.044 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.9 Å) |
Structure validation
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