4B1Q

NMR structure of the glycosylated conotoxin CcTx from Conus consors

Summary for 4B1Q

• Entry DOI: 10.2210/pdb4b1q/pdb
• NMR Information: BMRB: 18897
• Descriptor: CONOTOXIN CCTX, alpha-L-galactopyranose-(1-2)-beta-D-galactopyranose-(1-3)-[alpha-L-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-6)]2-acetamido-2-deoxy-alpha-D-galactopyranose (2 entities in total)
• Functional Keywords: toxin, o-glycan
• Biological source: CONUS CONSORS
• Cellular location: Secreted: P58928
• Total number of polymer chains: 1
• Total formula weight: 4142.57

Authors

Hocking, H.G.,Gerwig, G.J.,Favreau, P.,Stocklin, R.,Kamerling, J.P.,Boelens, R. (deposition date: 2012-07-12, release date: 2013-02-06, Last modification date: 2025-04-09)

Primary citation

Hocking, H.G.,Gerwig, G.J.,Dutertre, S.,Violette, A.,Favreau, P.,Stocklin, R.,Kamerling, J.P.,Boelens, R.
Structure of the O-Glycosylated Conopeptide Cctx from Conus Consors Venom.
Chemistry, 19:870-, 2013

PubMed Abstract: The glycopeptide CcTx, isolated from the venom of the piscivorous cone snail Conus consors, belongs to the κA-family of conopeptides. These toxins elicit excitotoxic responses in the prey by acting on voltage-gated sodium channels. The structure of CcTx, a first in the κA-family, has been determined by high-resolution NMR spectroscopy together with the analysis of its O-glycan at Ser7. A new type of glycopeptide O-glycan core structure, here registered as core type 9, containing two terminal L-galactose units {α-L-Galp-(1→4)-α-D-GlcpNAc-(1→6)-[α-L-Galp-(1→2)-β-D-Galp-(1→3)-]α-D-GalpNAc-(1→O)}, is highlighted. A sequence comparison to other putative members of the κA-family suggests that O-linked glycosylation might be more common than previously thought. This observation alone underlines the requirement for more careful and in-depth investigations into this type of post-translational modification in conotoxins.

PubMed: 23281027
DOI: 10.1002/CHEM.201202713

Experimental method

SOLUTION NMR