4AYJ

Molecular structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen

Summary for 4AYJ

• Entry DOI: 10.2210/pdb4ayj/pdb
• Related: 4AYL
• Related PRD ID: PRD_900070
• Descriptor: BOGT - METAL-INDEPENDENT GLYCOSYLTRANSFERASE, alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: transferase, catalysis
• Biological source: BACTEROIDES OVATUS
• Total number of polymer chains: 4
• Total formula weight: 118105.98

Authors

Thiyagarajan, N.,Pham, T.T.K.,Stinson, B.,Sundriyal, A.,Tumbale, P.,Lizotte-Waniewskib, M.,Brewb, K.,Acharya, K.R. (deposition date: 2012-06-21, release date: 2012-12-19, Last modification date: 2023-12-20)

Primary citation

Thiyagarajan, N.,Pham, T.T.K.,Stinson, B.,Sundriyal, A.,Tumbale, P.,Lizotte-Waniewski, M.,Brew, K.,Acharya, K.R.
Structure of a Metal-Independent Bacterial Glycosyltransferase that Catalyzes the Synthesis of Histo-Blood Group a Antigen
Sci.Rep., 2:940-, 2012

PubMed Abstract: Histo-blood group antigens (HBGAs) are a source of antigenic variation between individuals that modulates resistance and susceptibility to pathogens and is a barrier to the spread of enveloped viruses. HBGAs are also produced by a few prokaryotes where they are synthesized by glycosyltransferases (GTs) related to human HBGA synthases. Here we report the first structure of a bacterial GT of this family, from an intestinal resident, Bacteroides ovatus. Unlike its mammalian homologues and other GTs with similar folds, this protein lacks a metal-binding Asp-X-Asp motif and is fully active in the absence of divalent metal ions, yet is strikingly similar in structure and in its interactions with substrates to structurally characterized mammalian metal-dependent mammalian homologues. This shows how an apparently major divergence in catalytic properties can be accommodated by minor structural adjustments and illustrates the structural underpinnings of horizontal transfer of a functional gene from prokaryotes to vertebrates.

PubMed: 23230506
DOI: 10.1038/SREP00940

Experimental method

X-RAY DIFFRACTION (3 Å)