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4A20

Crystal structure of the Ubl domain of Mdy2 (Get5) at 1.78A

Summary for 4A20
Entry DOI10.2210/pdb4a20/pdb
Related2WPV
DescriptorUBIQUITIN-LIKE PROTEIN MDY2, SULFATE ION (3 entities in total)
Functional Keywordsprotein binding, get-pathway, tail-anchored proteins
Biological sourceSACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
Total number of polymer chains1
Total formula weight11404.99
Authors
Simon, A.C.,Simpson, P.J.,Murray, J.W.,Isaacson, R.L. (deposition date: 2011-09-21, release date: 2012-11-14, Last modification date: 2023-12-20)
Primary citationSimon, A.C.,Simpson, P.J.,Goldstone, R.M.,Krysztofinska, E.M.,Murray, J.W.,High, S.,Isaacson, R.L.
Structure of the Sgt2/Get5 Complex Provides Insights Into Get-Mediated Targeting of Tail-Anchored Membrane Proteins
Proc.Natl.Acad.Sci.USA, 110:1327-, 2013
Cited by
PubMed Abstract: Small, glutamine-rich, tetratricopeptide repeat protein 2 (Sgt2) is the first known port of call for many newly synthesized tail-anchored (TA) proteins released from the ribosome and destined for the GET (Guided Entry of TA proteins) pathway. This leads them to the residential membrane of the endoplasmic reticulum via an alternative to the cotranslational, signal recognition particle-dependent mechanism that their topology denies them. In yeast, the first stage of the GET pathway involves Sgt2 passing TA proteins on to the Get4/Get5 complex through a direct interaction between the N-terminal (NT) domain of Sgt2 and the ubiquitin-like (UBL) domain of Get5. Here we characterize this interaction at a molecular level by solving both a solution structure of Sgt2_NT, which adopts a unique helical fold, and a crystal structure of the Get5_UBL. Furthermore, using reciprocal chemical shift perturbation data and experimental restraints, we solve a structure of the Sgt2_NT/Get5_UBL complex, validate it via site-directed mutagenesis, and empirically determine its stoichiometry using relaxation experiments and isothermal titration calorimetry. Taken together, these data provide detailed structural information about the interaction between two key players in the coordinated delivery of TA protein substrates into the GET pathway.
PubMed: 23297211
DOI: 10.1073/PNAS.1207518110
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.78 Å)
Structure validation

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