488D

CATALYTIC RNA ENZYME-PRODUCT COMPLEX

488D の概要

• エントリーDOI: 10.2210/pdb488d/pdb
• 分子名称: RNA RIBOZYME STRAND, FIRST RNA FRAGMENT OF CLEAVED SUBSTRATE, SECOND RNA FRAGMENT OF CLEAVED SUBSTRATE, ... (5 entities in total)
• 機能のキーワード: catalytic rna, ribozyme, enzyme-product complex, crystal lattice trapping, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 22126.15

構造登録者

Murray, J.B.,Szoke, H.,Szoke, A.,Scott, W.G. (登録日: 2000-02-25, 公開日: 2000-03-06, 最終更新日: 2023-12-27)

主引用文献

Murray, J.B.,Szoke, H.,Szoke, A.,Scott, W.G.
Capture and visualization of a catalytic RNA enzyme-product complex using crystal lattice trapping and X-ray holographic reconstruction.
Mol.Cell, 5:279-287, 2000

PubMed Abstract: We have determined the crystal structure of the enzyme-product complex of the hammerhead ribozyme by using a reinforced crystal lattice to trap the complex prior to dissociation and by employing X-ray holographic image reconstruction, a real-space electron density imaging and refinement procedure. Subsequent to catalysis, the cleavage site residue (C-17), together with its 2',3'-cyclic phosphate, adopts a conformation close to and approximately perpendicular to the Watson-Crick base-pairing faces of two highly conserved purines in the ribozyme's catalytic pocket (G-5 and A-6). We observe several interactions with functional groups on these residues that have been identified as critical for ribozyme activity by biochemical analyses but whose role has defied explanation in terms of previous structural analyses. These interactions may therefore be relevant to the hammerhead ribozyme reaction mechanism.

PubMed: 10882069
DOI: 10.1016/S1097-2765(00)80423-2

実験手法

X-RAY DIFFRACTION (3.1 Å)