425D

5'-D(*AP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*T)-3'

425D の概要

• エントリーDOI: 10.2210/pdb425d/pdb
• 分子名称: DNA (5'-D(*AP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*T)-3') (2 entities in total)
• 機能のキーワード: double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7326.78

構造登録者

Rozenberg, H.,Rabinovich, D.,Frolow, F.,Hegde, R.S.,Shakked, Z. (登録日: 1998-09-14, 公開日: 1999-10-14, 最終更新日: 2024-04-03)

主引用文献

Rozenberg, H.,Rabinovich, D.,Frolow, F.,Hegde, R.S.,Shakked, Z.
Structural code for DNA recognition revealed in crystal structures of papillomavirus E2-DNA targets.
Proc.Natl.Acad.Sci.USA, 95:15194-15199, 1998

PubMed Abstract: Transcriptional regulation in papillomaviruses depends on sequence-specific binding of the regulatory protein E2 to several sites in the viral genome. Crystal structures of bovine papillomavirus E2 DNA targets reveal a conformational variant of B-DNA characterized by a roll-induced writhe and helical repeat of 10.5 bp per turn. A comparison between the free and the protein-bound DNA demonstrates that the intrinsic structure of the DNA regions contacted directly by the protein and the deformability of the DNA region that is not contacted by the protein are critical for sequence-specific protein/DNA recognition and hence for gene-regulatory signals in the viral system. We show that the selection of dinucleotide or longer segments with appropriate conformational characteristics, when positioned at correct intervals along the DNA helix, can constitute a structural code for DNA recognition by regulatory proteins. This structural code facilitates the formation of a complementary protein-DNA interface that can be further specified by hydrogen bonds and nonpolar interactions between the protein amino acids and the DNA bases.

PubMed: 9860945
DOI: 10.1073/pnas.95.26.15194

実験手法

X-RAY DIFFRACTION (2.8 Å)