414D

5'-D(*GP*GP*GP*GP*CP*GP*CP*CP*CP*C)-3'

414D の概要

• エントリーDOI: 10.2210/pdb414d/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*GP*CP*GP*CP*CP*CP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna, double helix, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12187.92

構造登録者

Savitha, G.,Viswamitra, M.A. (登録日: 1998-07-13, 公開日: 1999-06-14, 最終更新日: 2024-02-28)

主引用文献

Savitha, G.,Viswamitra, M.A.
An A-DNA structure with two independent duplexes in the asymmetric unit.
Acta Crystallogr.,Sect.D, 55:1136-1143, 1999

PubMed Abstract: The crystal and molecular structure of the self-complementary A-DNA decamer sequence d(G4CGC4) was solved at 1.9 A resolution. The decamer crystallizes in space group P21 with two independent duplexes in the asymmetric unit. Duplex 1 has interactions which are distributed symmetrically about its length compared with duplex 2. The two end base pairs of duplex 1 have a similar NH.O hydrogen-bond pattern involving GGC segments of duplex 2 and a symmetry-related neighbour, while the end base pairs of duplex 2 interact with the GCC and GGG segments of its symmetry-related neighbours through NH.O and NH.N hydrogen bonds and a water-mediated hydrogen bond between the carboxyl groups of C40 and C8. In addition to the C4'-C5' torsion angle gamma assuming the trans conformation in certain steps, this angle also adopts the gauche- conformation at C37 as opposed to the preferred gauche+ conformation, with a concomitant change in phosphodiester P-O5' (alpha) in the opposite sense. This facilitates stacking between adjacent bases. The study suggests that the structural alterations in the two molecules in the asymmetric unit originate from an inherent propensity of the d(G4CGC4) base sequence for varied intermolecular interactions and malleability.

PubMed: 10329775
DOI: 10.1107/S0907444999003182

実験手法

X-RAY DIFFRACTION (1.9 Å)