3ZZS

Engineered 12-subunit Bacillus stearothermophilus trp RNA-binding attenuation protein (TRAP)

3ZZS の概要

• エントリーDOI: 10.2210/pdb3zzs/pdb
• 関連するPDBエントリー: 1C9S 1GTF 1GTN 1QAW 1UTD 1UTF 1UTV
• 分子名称: TRANSCRIPTION ATTENUATION PROTEIN MTRB, TRYPTOPHAN (3 entities in total)
• 機能のキーワード: transcription, transcription regulation, protein engineering
• 由来する生物種: GEOBACILLUS STEAROTHERMOPHILUS
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 66783.79

構造登録者

Chen, C.,Smits, C.,Dodson, G.G.,Shevtsov, M.B.,Merlino, N.,Gollnick, P.,Antson, A.A. (登録日: 2011-09-02, 公開日: 2011-10-12, 最終更新日: 2023-12-20)

主引用文献

Chen, C.,Smits, C.,Dodson, G.G.,Shevtsov, M.B.,Merlino, N.,Gollnick, P.,Antson, A.A.
How to Change the Oligomeric State of a Circular Protein Assembly: Switch from 11-Subunit to 12-Subunit Trap Suggests a General Mechanism
Plos One, 6:25296-, 2011

PubMed Abstract: Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligomeric states has prevented engineering such transitions. In this study we used the bacterial transcription regulator TRAP as a model system to investigate the features that define the oligomeric state of a circular protein and to question how the subunit number could be manipulated.

PubMed: 21984911
DOI: 10.1371/JOURNAL.PONE.0025296

実験手法

X-RAY DIFFRACTION (1.49 Å)