3ZYT

Structure Determination of EstA from Arthrobacter nitroguajacolicus Rue61a

3ZYT の概要

• エントリーDOI: 10.2210/pdb3zyt/pdb
• 分子名称: ESTERASE A, ETHYL MERCURY ION (3 entities in total)
• 機能のキーワード: hydrolase, enzymatic promiscuity, beta-lactamase
• 由来する生物種: ARTHROBACTER NITROGUAJACOLICUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40312.69

構造登録者

Wagner, U.G.,Fetzner, S. (登録日: 2011-08-25, 公開日: 2012-09-19, 最終更新日: 2024-05-08)

主引用文献

Wagner, U.G.,Dimaio, F.,Kolkenbrock, S.,Fetzner, S.
Crystal Structure Analysis of Esta from Arthrobacter Sp. Rue61A--an Insight Into Catalytic Promiscuity.
FEBS Lett., 588:1154-, 2014

PubMed Abstract: In this article we analyze the reasons for catalytic promiscuity of a type VIII esterase with β-lactamase fold and the ability to cleave β-lactams. We compared the structure of this enzyme to those of an esterase of the same type without any lactamase ability, an esterase with moderate lactamase ability, and a class C β-lactamase with similar fold. Our results show that for these enzymes, the difference in the substrate specificity is sterically driven.

PubMed: 24613918
DOI: 10.1016/J.FEBSLET.2014.02.045

実験手法

X-RAY DIFFRACTION (2.45 Å)