3ZXO

CRYSTAL STRUCTURE OF THE MUTANT ATP-BINDING DOMAIN OF MYCOBACTERIUM TUBERCULOSIS DOSS

3ZXO の概要

• エントリーDOI: 10.2210/pdb3zxo/pdb
• 関連するPDBエントリー: 2W3D 2W3E 2W3F 2W3G 2W3H 2Y79 3ZXQ
• 分子名称: REDOX SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS, ZINC ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: MYCOBACTERIUM TUBERCULOSIS
• 細胞内の位置: Cytoplasm (Probable): P95194
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27892.65

構造登録者

Cho, H.Y.,Cho, H.J.,Kang, B.S. (登録日: 2011-08-13, 公開日: 2011-08-24, 最終更新日: 2025-04-09)

主引用文献

Cho, H.Y.,Lee, Y.H.,Bae, Y.S.,Kim, E.,Kang, B.S.
Activation of ATP Binding for the Autophosphorylation of Doss, a Mycobacterium Tuberculosis Histidine Kinase Lacking an ATP-Lid Motif.
J.Biol.Chem., 288:12437-, 2013

PubMed Abstract: The sensor histidine kinases of Mycobacterium tuberculosis, DosS and DosT, are responsible for sensing hypoxic conditions and consist of sensor and kinase cores responsible for accepting signals and phosphorylation activity, respectively. The kinase core contains a dimerization and histidine phosphate-accepting (DHp) domain and an ATP binding domain (ABD). The 13 histidine kinase genes of M. tuberculosis can be grouped based on the presence or absence of the ATP lid motif and F box (elements known to play roles in ATP binding) in their ABDs; DosS and DosT have ABDs lacking both these elements, and the crystal structures of their ABDs indicated that they were unsuitable for ATP binding, as a short loop covers the putative ATP binding site. Although the ABD alone cannot bind ATP, the kinase core is functional in autophosphorylation. Appropriate spatial arrangement of the ABD and DHp domain within the kinase core is required for both autophosphorylation and ATP binding. An ionic interaction between Arg(440) in the DHp domain and Glu(537) in the short loop of the ABD is available and may open the ATP binding site, by repositioning the short loop away from the site. Mutations at Arg(440) and Glu(537) reduce autophosphorylation activity. Unlike other histidine kinases containing an ATP lid, which protects bound ATP, DosS is unable to accept ATP until the ABD is properly positioned relative to the histidine; this may prevent unexpected ATP reactions. ATP binding can, therefore, function as a control mechanism for histidine kinase activity.

PubMed: 23486471
DOI: 10.1074/JBC.M112.442467

実験手法

X-RAY DIFFRACTION (1.9 Å)