3ZXB

Wild type single insulin-like growth factor binding domain protein (SIBD-1) from Cupiennius salei

Summary for 3ZXB

• Entry DOI: 10.2210/pdb3zxb/pdb
• Related: 3ZXC
• Descriptor: SINGLE INSULIN-LIKE GROWTH FACTOR-BINDING DOMAIN PROTEIN-1 (2 entities in total)
• Functional Keywords: signaling, igfbp, insulin binding domain
• Biological source: CUPIENNIUS SALEI
• Total number of polymer chains: 4
• Total formula weight: 32668.81

Authors

Widmer, C.,Baumann, U. (deposition date: 2011-08-09, release date: 2012-06-06, Last modification date: 2023-12-20)

Primary citation

Trachsel, C.,Widmer, C.,Kampfer, U.,Buhr, C.,Baumann, T.,Kuhn-Nentwig, L.,Schurch, S.,Schaller, J.,Baumann, U.
Structural and Biochemical Characterization of Native and Recombinant Single Insulin-Like Growth Factor-Binding Domain Protein (Sibd-1) from the Central American Hunting Spider Cupiennius Salei (Ctenidae).
Proteins, 80:2323-, 2012

PubMed Abstract: Cupiennius salei single insulin-like growth factor binding domain protein (SIBD-1) is an 8.6 kDa Cys-, Pro-, and Gly-rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei. SIBD-1 exhibits high sequence similarity to the N-terminal domain of the insulin-like growth factor-binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three-dimensional structure of recombinant SIBD-1 and the characterization of the sugar moiety at Thr2 of native SIBD-1 is described in detail.

PubMed: 22622866
DOI: 10.1002/PROT.24119

Experimental method

X-RAY DIFFRACTION (2.55 Å)