3ZWJ

CRYSTAL STRUCTURE OF THE PORE-FORMING TOXIN FRAC FROM ACTINIA FRAGACEA (Form 3)

3ZWJ の概要

• エントリーDOI: 10.2210/pdb3zwj/pdb
• 関連するPDBエントリー: 3ZWG
• 分子名称: FRAGACEATOXIN C (2 entities in total)
• 機能のキーワード: toxin, membrane proteins, pore-forming toxins, actinoporins
• 由来する生物種: ACTINIA FRAGACEA
• 細胞内の位置: Secreted (By similarity): B9W5G6
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 177716.73

構造登録者

Mechaly, A.E.,Bellomioa, A.,Morantea, K.,Gonzalez-Manas, J.M.,Guerin, D.M.A. (登録日: 2011-08-01, 公開日: 2012-07-04, 最終更新日: 2023-12-20)

主引用文献

Mechaly, A.E.,Bellomio, A.,Morante, K.,Agirre, J.,Gil-Carton, D.,Valle, M.,Gonzalez-Manas, J.M.,Guerin, D.M.A.
Pores of the Toxin Frac Assemble Into 2D Hexagonal Clusters in Both Crystal Structures and Model Membranes.
J.Struct.Biol., 180:312-, 2012

PubMed Abstract: The recent high-resolution structure of the toxin FraC derived from the sea anemone Actinia fragacea has provided new insight into the mechanism of pore formation by actinoporins. In this work, we report two new crystal forms of FraC in its oligomeric prepore conformation. Together with the previously reported structure, these two new structures reveal that ring-like nonamers of the toxin assemble into compact two-dimensional hexagonal arrays. This supramolecular organization is maintained in different relative orientations adopted by the oligomers within the crystal layers. Analyses of the aggregation of FraC pores in both planar and curved (vesicles) model membranes show similar 2D hexagonal arrangements. Our observations support a model in which hexagonal pore-packing is a clustering mechanism that maximizes toxin-driven membrane damage in the target cell.

PubMed: 22728830
DOI: 10.1016/J.JSB.2012.06.003

実験手法

X-RAY DIFFRACTION (2.37 Å)