3ZTN

STRUCTURE OF INFLUENZA A NEUTRALIZING ANTIBODY SELECTED FROM CULTURES OF SINGLE HUMAN PLASMA CELLS IN COMPLEX WITH HUMAN H1 INFLUENZA HAEMAGGLUTININ.

Summary for 3ZTN

• Entry DOI: 10.2210/pdb3ztn/pdb
• Descriptor: HAEMAGGLUTININ, FI6V3 ANTIBODY LIGHT CHAIN, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: viral protein-immune system complex, monoclonal antibody., viral protein/immune system
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 4
• Total formula weight: 107635.79

Authors

Hubbard, P.A.,Ritchie, A.J.,Corti, D.,Voss, J.E.,Gamblin, S.J.,Codoni, G.,Macagno, A.,Jarrossay, D.,Pinna, D.,Minola, A.,Vanzetta, F.,Silacci, C.,Fernandez-Rodriguez, B.M.,Agatic, G.,Giacchetto-Sasselli, I.,Vachieri, S.G.,Sallusto, F.,Collins, P.J.,Haire, L.F.,Temperton, N.,Langedijk, J.P.M.,Skehel, J.J.,Lanzavecchia, A. (deposition date: 2011-07-12, release date: 2011-08-10, Last modification date: 2023-12-20)

Primary citation

Corti, D.,Voss, J.E.,Gamblin, S.J.,Codoni, G.,Macagno, A.,Jarrossay, D.,Vachieri, S.G.,Pinna, D.,Minola, A.,Vanzetta, F.,Silacci, C.,Fernandez-Rodriguez, B.M.,Agatic, G.,Bianchi, S.,Giacchetto-Sasselli, I.,Calder, L.,Sallusto, F.,Collins, P.J.,Haire, L.F.,Temperton, N.,Langedijk, J.P.M.,Skehel, J.J.,Lanzavecchia, A.
A Neutralizing Antibody Selected from Plasma Cells that Binds to Group 1 and Group 2 Influenza a Hemagglutinins.
Science, 333:850-, 2011

PubMed Abstract: The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

PubMed: 21798894
DOI: 10.1126/SCIENCE.1205669

Experimental method

X-RAY DIFFRACTION (3.001 Å)