3ZQS

Human FANCL central domain

3ZQS の概要

• エントリーDOI: 10.2210/pdb3zqs/pdb
• 分子名称: E3 UBIQUITIN-PROTEIN LIGASE FANCL, PROLINE, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: ligase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: Q9NW38
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45309.73

構造登録者

Hodson, C.,Cole, A.R.,Purkiss-Trew, A.,Walden, H. (登録日: 2011-06-10, 公開日: 2011-07-20, 最終更新日: 2023-12-20)

主引用文献

Hodson, C.,Cole, A.R.,Lewis, L.P.,Miles, J.A.,Purkiss-Trew, A.,Walden, H.
Structural Analysis of Human Fancl, the E3 Ligase in the Fanconi Anemia Pathway.
J.Biol.Chem., 286:32628-, 2011

PubMed Abstract: The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand cross-links. At the heart of this pathway is the monoubiquitination of the FANCI-FANCD2 (ID) complex by the multiprotein "core complex" containing the E3 ubiquitin ligase FANCL. Vertebrate organisms have the eight-protein core complex, whereas invertebrates apparently do not. We report here the structure of the central domain of human FANCL in comparison with the recently solved Drosophila melanogaster FANCL. Our data represent the first structural detail into the catalytic core of the human system and reveal that the central fold of FANCL is conserved between species. However, there are macromolecular differences between the FANCL proteins that may account for the apparent distinctions in core complex requirements between the vertebrate and invertebrate FA pathways. In addition, we characterize the binding of human FANCL with its partners, Ube2t, FANCD2, and FANCI. Mutational analysis reveals which residues are required for substrate binding, and we also show the domain required for E2 binding.

PubMed: 21775430
DOI: 10.1074/JBC.M111.244632

実験手法

X-RAY DIFFRACTION (2 Å)