3ZO5

Structure of SENP2-Loop1 in complex with preSUMO-2

3ZO5 の概要

• エントリーDOI: 10.2210/pdb3zo5/pdb
• 分子名称: SENTRIN-SPECIFIC PROTEASE 2, SMALL UBIQUITIN-RELATED MODIFIER 2 (3 entities in total)
• 機能のキーワード: hydrolase-signaling protein complex, hydrolase/signaling protein
• 由来する生物種: HOMO SAPIENS; 詳細
• 細胞内の位置: Nucleus, nuclear pore complex: Q9HC62; Nucleus: P61956
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38119.73

構造登録者

Alegre, K.O.,Reverter, D. (登録日: 2013-02-20, 公開日: 2014-01-29, 最終更新日: 2023-12-20)

主引用文献

Alegre, K.O.,Reverter, D.
Structural Insights Into the Senp6 Loop1 Structure in Complex with Sumo2.
Protein Sci., 23:433-, 2014

PubMed Abstract: The SENP proteases regulate the SUMO conjugates in the cell by cleaving SUMO from target proteins. SENP6 and SENP7 are the most divergent members of the SENP/ULP protease family in humans by the presence of insertions in their catalytic domains. Loop1 insertion is determinant for the SUMO2/3 activity and specificity on SENP6 and SENP7. To gain structural insights into the role of Loop1, we have designed a chimeric SENP2 with the insertion of Loop1 into its sequence. The structure of SENP2-Loop1 in complex with SUMO2 was solved at 2.15 Å resolution, and reveals the details of an interface exclusive to SENP6/7 and the formation of unique contacts between both proteins. Interestingly, functional data with SUMO substrates showed an increase of the proteolytic activity in the SENP2-Loop1 chimera for diSUMO2 and polySUMO2 substrates.

PubMed: 24424631
DOI: 10.1002/PRO.2425

実験手法

X-RAY DIFFRACTION (2.15 Å)