3ZBJ

Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

Summary for 3ZBJ

• Entry DOI: 10.2210/pdb3zbj/pdb
• Related: 3ZBI
• EMDB information: 2233
• Descriptor: TRAO PROTEIN (1 entity in total)
• Functional Keywords: cell adhesion, bacterial secretion
• Biological source: ESCHERICHIA COLI
• Total number of polymer chains: 14
• Total formula weight: 175885.01

Authors

Rivera-Calzada, A.,Fronzes, R.,Savva, C.G.,Chandran, V.,Lian, P.W.,Laeremans, T.,Pardon, E.,Steyaert, J.,Remaut, H.,Waksman, G.,Orlova, E.V. (deposition date: 2012-11-10, release date: 2013-04-03, Last modification date: 2024-05-08)

Primary citation

Rivera-Calzada, A.,Fronzes, R.,Savva, C.G.,Chandran, V.,Lian, P.W.,Laeremans, T.,Pardon, E.,Steyaert, J.,Remaut, H.,Waksman, G.,Orlova, E.V.
Structure of a Bacterial Type Iv Secretion Core Complex at Subnanometre Resolution.
Embo J., 32:1195-, 2013

PubMed Abstract: Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

PubMed: 23511972
DOI: 10.1038/EMBOJ.2013.58

Experimental method

ELECTRON MICROSCOPY (8.5 Å)