3WYP

Crystal structure of wild-type core streptavidin in complex with D-biotin/biotin-D-sulfoxide at 1.3 A resolution

3WYP の概要

• エントリーDOI: 10.2210/pdb3wyp/pdb
• 関連するPDBエントリー: 3WYQ
• 分子名称: Streptavidin, BIOTIN, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: beta-barrel, biotin binding protein
• 由来する生物種: Streptomyces avidinii
• 細胞内の位置: Secreted: P22629
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 55247.65

構造登録者

Kawato, T.,Mizohata, E.,Meshizuka, T.,Doi, H.,Kawamura, T.,Matsumura, H.,Yumura, K.,Tsumoto, K.,Kodama, T.,Inoue, T.,Sugiyama, A. (登録日: 2014-09-05, 公開日: 2014-12-24, 最終更新日: 2023-11-08)

主引用文献

Kawato, T.,Mizohata, E.,Meshizuka, T.,Doi, H.,Kawamura, T.,Matsumura, H.,Yumura, K.,Tsumoto, K.,Kodama, T.,Inoue, T.,Sugiyama, A.
Crystal structure of streptavidin mutant with low immunogenicity.
J.Biosci.Bioeng., 119:642-647, 2015

PubMed Abstract: We previously created a low-immunogenic core streptavidin mutant No. 314 (LISA-314) by replacing six amino-acid residues for use as a delivery tool for an antibody multistep pre-targeting process (Yumura et al., Protein Sci., 22, 213-221, 2013). Here, we performed high-resolution X-ray structural analyses of LISA-314 and wild-type streptavidin to investigate the effect of substitutions on the protein function and the three-dimensional structure. LISA-314 forms a tetramer in the same manner as wild-type streptavidin. The binding mode of d-biotin in LISA-314 is also completely identical to that in wild-type streptavidin, and conformational changes were observed mostly at the side chains of substituted sites. Any large conformational changes corresponding to the reduction of B factors around the substituted sites were not observed. These results demonstrated the LISA-314 acquired low immunogenicity without losing structural properties of original wild-type streptavidin.

PubMed: 25434833
DOI: 10.1016/j.jbiosc.2014.10.025

実験手法

X-RAY DIFFRACTION (1.3 Å)