3WY7

Crystal structure of Mycobacterium smegmatis 7-Keto-8-aminopelargonic acid (KAPA) synthase BioF

3WY7 の概要

• エントリーDOI: 10.2210/pdb3wy7/pdb
• 分子名称: 8-amino-7-oxononanoate synthase (2 entities in total)
• 機能のキーワード: domain swapping, alpha and beta, alpha-beta-alpha sandwich, synthase, pyridoxal 5'-phosphate (plp) binding, transferase
• 由来する生物種: Mycobacterium smegmatis str. MC2 155
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 167729.62

構造登録者

Fan, S.H.,Li, D.F.,Wang, D.C.,Chen, G.J.,Zhang, X.E.,Bi, L.J. (登録日: 2014-08-20, 公開日: 2014-12-17, 最終更新日: 2023-11-08)

主引用文献

Fan, S.H.,Li, D.F.,Wang, D.C.,Fleming, J.,Zhang, H.T.,Zhou, Y.,Zhou, L.,Zhou, J.,Chen, T.,Chen, G.J.,Zhang, X.E.,Bi, L.J.
Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase
Int.J.Biochem.Cell Biol., 58C:71-80, 2014

PubMed Abstract: The biotin biosynthesis pathway is an attractive target for development of novel drugs against mycobacterial pathogens, however there are as yet no suitable inhibitors that target this pathway in mycobacteria. 7-Keto-8-aminopelargonic acid synthase (KAPA synthase, BioF) is the enzyme which catalyzes the first committed step of the biotin synthesis pathway, but both its structure and function in mycobacteria remain unresolved. Here we present the crystal structure of Mycobacterium smegmatis BioF (MsBioF). The structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS), the E. coli homologue of BioF. To investigate the influence of structural characteristics on the function of MsBioF, we deleted bioF in M. smegmatis and confirmed that BioF is required for growth in the absence of exogenous biotin. Based on structural and mutagenesis studies, we confirmed that pyridoxal 5'-phosphate (PLP) binding site residues His129, Lys235 and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo. The structure and function of MsBioF reported here provides further insights for developing new anti-tuberculosis inhibitors aimed at the biotin synthesis pathway.

PubMed: 25462832
DOI: 10.1016/j.biocel.2014.11.006

実験手法

X-RAY DIFFRACTION (2.3 Å)