3WXS

Thaumatin structure determined by SPring-8 Angstrom Compact free electron Laser (SACLA)

3WXS の概要

• エントリーDOI: 10.2210/pdb3wxs/pdb
• 関連するPDBエントリー: 3al7 3ald 3aok 3vhf 3wou
• 分子名称: thaumatin I, L(+)-TARTARIC ACID (3 entities in total)
• 機能のキーワード: thaumatin, sweet-tasting protein, thaumatin family, sweet receptor, mainly beta, taste protein, aril, plant protein
• 由来する生物種: Thaumatococcus daniellii (katemfe, miracle fruit)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22378.13

構造登録者

Masuda, T.,Nango, E.,Sugahara, M.,Mizohata, E.,Tanaka, T.,Tanaka, R.,Suzuki, M.,Mikami, B.,Iwata, S. (登録日: 2014-08-07, 公開日: 2014-11-05, 最終更新日: 2024-11-20)

主引用文献

Sugahara, M.,Mizohata, E.,Nango, E.,Suzuki, M.,Tanaka, T.,Masuda, T.,Tanaka, R.,Shimamura, T.,Tanaka, Y.,Suno, C.,Ihara, K.,Pan, D.,Kakinouchi, K.,Sugiyama, S.,Murata, M.,Inoue, T.,Tono, K.,Song, C.,Park, J.,Kameshima, T.,Hatsui, T.,Joti, Y.,Yabashi, M.,Iwata, S.
Grease matrix as a versatile carrier of proteins for serial crystallography
Nat. Methods, 12:61-63, 2015

PubMed Abstract: Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

PubMed: 25384243
DOI: 10.1038/nmeth.3172

実験手法

X-RAY DIFFRACTION (2 Å)