3WVU

Crystal Structure of RuCO/apo-R52CFr

3WVU の概要

• エントリーDOI: 10.2210/pdb3wvu/pdb
• 関連するPDBエントリー: 3WVV 3WVW
• 分子名称: Ferritin light chain, CADMIUM ION, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: protein structure initiative, metal binding protein
• 由来する生物種: Equus caballus (domestic horse,equine)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20950.36

構造登録者

Fujita, K.,Tanaka, Y.,Abe, S.,Hikage, T.,Kuchimaru, T.,Kizaka-Kondoh, S.,Ueno, T. (登録日: 2014-06-09, 公開日: 2015-07-15, 最終更新日: 2024-03-20)

主引用文献

Fujita, K.,Tanaka, Y.,Sho, T.,Ozeki, S.,Abe, S.,Hikage, T.,Kuchimaru, T.,Kizaka-Kondoh, S.,Ueno, T.
Intracellular CO release from composite of ferritin and ruthenium carbonyl complexes.
J.Am.Chem.Soc., 136:16902-16908, 2014

PubMed Abstract: Protein cages have been utilized as templates in the development of biomaterials. Here we report protein engineering of the ferritin (Fr) cage for encapsulating carbon monoxide releasing molecules (CORMs) and release of CO gas which serves as a cell signaling molecule. The protein cages enable us to increase the half-life for CO release, providing a release rate that is 18-fold slower than the rate of a typical CORM, Ru(CO)3Cl(glycinate) (CORM-3). Moreover, the uptake ratio of the composite is about 4-fold greater than that of CORM-3. We found that these effects enhance the activation of nuclear factor κB 10-fold higher than CORM-3. The protein cage of Fr thus provides the basis for new CORMs that can be used for in vitro cell research.

PubMed: 25352251
DOI: 10.1021/ja508938f

実験手法

X-RAY DIFFRACTION (1.92 Å)