3WV0

O-glycan attached to herpes simplex virus type 1 glycoprotein gB is recognized by the Ig V-set domain of human paired immunoglobulin-like type 2 receptor alpha

3WV0 の概要

• エントリーDOI: 10.2210/pdb3wv0/pdb
• 関連するPDBエントリー: 3WUZ
• 分子名称: Paired immunoglobulin-like type 2 receptor alpha, Envelope glycoprotein B, N-acetyl-alpha-neuraminic acid-(2-6)-2-acetamido-2-deoxy-alpha-D-galactopyranose, ... (5 entities in total)
• 機能のキーワード: immunoglobulin-like, immunological receptor, membrane, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Virion membrane ; Single-pass type I membrane protein : P06437
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 30948.17

構造登録者

Kuroki, K.,Wang, J.,Ose, T.,Yamaguchi, M.,Tabata, S.,Maita, N.,Nakamura, S.,Kajikawa, M.,Kogure, A.,Satoh, T.,Arase, H.,Maenaka, K. (登録日: 2014-05-10, 公開日: 2014-06-11, 最終更新日: 2024-11-20)

主引用文献

Kuroki, K.,Wang, J.,Ose, T.,Yamaguchi, M.,Tabata, S.,Maita, N.,Nakamura, S.,Kajikawa, M.,Kogure, A.,Satoh, T.,Arase, H.,Maenaka, K.
Structural basis for simultaneous recognition of an O-glycan and its attached peptide of mucin family by immune receptor PILR alpha
Proc.Natl.Acad.Sci.USA, 111:8877-8882, 2014

PubMed Abstract: Paired Ig-like type 2 receptor α (PILRα) recognizes a wide range of O-glycosylated mucin and related proteins to regulate broad immune responses. However, the molecular characteristics of these recognitions are largely unknown. Here we show that sialylated O-linked sugar T antigen (sTn) and its attached peptide region are both required for ligand recognition by PILRα. Furthermore, we determined the crystal structures of PILRα and its complex with an sTn and its attached peptide region. The structures show that PILRα exhibits large conformational change to recognize simultaneously both the sTn O-glycan and the compact peptide structure constrained by proline residues. Binding and functional assays support this binding mode. These findings provide significant insight into the binding motif and molecular mechanism (which is distinct from sugar-recognition receptors) by which O-glycosylated mucin proteins with sTn modifications are recognized in the immune system as well as during viral entry.

PubMed: 24889612
DOI: 10.1073/pnas.1324105111

実験手法

X-RAY DIFFRACTION (2.3 Å)